Endophytes Colonize
Poster presented by De’Jana Parker at the 2017 Plant Meeting in Oahu, Hawaii
experimental
First Year Conclusions and Directions
We had a very successful year. It is worth noting that this project did not exist a year ago. The hard work of the Tague lab crew is evident. We …
Project 6) Does size matter with the modified protocol?
Endophyte 3.1 (BWT) ANSWER: Smaller is better, but the 2.5 cork borer may be the best to use for consistent results. The modified protocol is fine, but it is more …
Project 5) Is the sterilization protocol too harsh?
Endophyte 3.0 (SH, AQ, more?) Answer: Maybe. Experiment We compared our Standard protocol to a modified protocol. From the same leaf, we 1) cut disks → surface sterilize as usual → plated AND …
Project 4) Does size (of the the piece of leaf tissue) matter?
Endophyte 2.0 Answer: Yes! But in a different way than Gamboa et al (2002). The population density of culturable fungi in our (temperate) Magnolia seems to be much lower than …
Project 3) Can we store and recover fungi for long term storage?
Answer: Yes! Experiment Growing tips of fungi emerging from leaf tissue is excised and transplanted to a new culture plate. If culture is pure (by visual inspection), thrice-autoclaved barley seeds …
Project 2) Can we culture any endophytes from Magnolia leaf tissue?
Endophyte 1.0 (Fall semester- early Spring 2014) ANSWER: YES! We see some morphotypes consistently emerging from Magnolia leaf tissue. Experiment: Our first experiments were exploratory in nature. We adapted published …
Project 1) What’s in the Air?
ANSWER: All kinds of stuff, but very different from those we find in Magnolia. Experiment: MEA (malt extract agar) or PDA (potato dextrose agar) plates were left open for …